PRODUCTS

Gelatin/Collagen for Tissue Engineering

beMatrix

beMatrix gelatin is a series of highly safety collagen and gelatin. For all products, endotoxin reduction and virus inactivation treatment are carried out.

Low Endotoxin Gelatin
Expansion to medical field

Low Endotoxin Gelatin

USP: Low endotoxin level (NMT 10 EU/g)

■ Storage condition: Room temperature

■ Expiration date: Three years from the date of
manufacture

Gelatin LS-H

・Alkaline-treated gelatin derived from porcine skin.

・High Gel strength.

Gelatin LS-W

・Alkaline-treated gelatin derived from porcine skin.

・Low Gel strength.

■Safety test

A safety test was conducted and the product was confirmed to be negative to the follow-ing five items (cytotoxicity test, sensitization test, intradermal reaction test, pyrogen test, and antigenicity test).

Item Result
Cytotoxicity test Negative
Sensitization test (guinea pig) Negative
Intradermal reaction test (rabbit) Negative
Pyrogen test (rabbit) Negative
Antigenicity test (guinea pig) Negative

■Usage example

The product and a crosslinking agent were used to prepare a hydrogel with a water contentof 96 %, Histological section (a and c), which were made from the hydrogel incorporating pla-telet rich plasma (PRP) with a basic fibroblast growth factor (bFGF), and specimens (b and d),which were made from the hydrogel incorporating with phosphate buffered saline (PBS), wereinjected in the location of ischemia in the lower limbs of the mice for a week. Then the subse-quent angiogenesis was confirmed. Section a) and b) show HE-staining and Section c) and d)show a-smooth muscle actin (a-SMA) immunohisto chemical staining. It is possible to usethis product as a carrier for drug delivery system. *From reference 17).

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■References

1) Horinaka, J., et al. Int J Biol Macromol 2016; 91: 789
2) Koshy, ST., et al. Adv Healthc Mater 2016; 5: 541
3) Yoshizawa, K., et al. Biomaterials 2015; 63: 14
4) Morimoto, N., et al. BMJ Open 2015; 5: e007733
5) Kumagai, M., et al. Heart and Vessels 2015; 11: 1
6) Ishimaru, T., et al. J Pediatr Surg 2015; 50: 255
7) Hoshi, S., et al. J Periodontal Res 2015; Early View: jre.12285
8) Ajioka, I., et al. Tissue Eng Part A 2015; 21: 193
9) Kabuto, Y., et al. Tissue Eng Part A 2015; 21: 2025
10) Kimoto, M., et al. Invest Ophthalmol Vis Sci 2014; 55: 2337
11) Yoshizawa, K., et al. J Bioact Compat Polym 2014; 29: 560
12) Inoue, M., et al. J Biomater Appl 2014; 28: 880

13) Ohyabu, Y., et al. J Biosci Bioeng 2014; 118: 112
14) Yoshizawa, K., et al. Int J Mol Sci 2014; 15: 2142
15) Kiminami, K., et al. Key Eng Mater 2014; 631: 397
16) Inoue, M., et al. J Biomed Mater Res A 2013; 101: 2049
17) Matsui, M., et al. Acta Biomater 2012; 8: 1792
18) Inoue, M., et al. Adv Healthc Mater 2012; 1: 573
19) Inoue, M., et al. Sci Technol Adv Mater 2012; 13: 064215
20) Inoue, M., et al. Colloids Surf B Biointerfaces 2011; 88: 260
21) Tara, S., et al. Geriatr Gerontol Int 2011; 11: 527
22) Nakagawa, T., et al. BMC Medicine 2010; 8: 76
23) Kawanaka, H., et al. Am J Med Sci 2009; 338: 341

Low Endotoxin Collagen

Low Endotoxin Collagen

USP:Low endotoxin level (NMT 0.5EU/mL)
■Storage condition: Cold storage (4℃ to 8℃)
■Expiration date: Two years from the date of manufacture

Collagen AT

Acid extracted collagen solution derived from porcine tendon

■USP: Low endotoxin level (NMT 0.5 EU/mL)

■Storage condition: Cold storage (4 °C to 8 °C)

■Expiration date: Two years from the date of manufacture

・Concentration: 3 mg/mL, pH3

Collagen TE

Pepsin-solubilized collagen solution derived from porcine skin

■USP: Low endotoxin level (NMT 0.5 EU/mL)

■Storage condition: Cold storage (4 °C to 8 °C)

■Expiration date: One year from the date of manufacture

・Concentration: 5 mg/mL, pH3

Collagen FD

Freeze-dried atelocollagen derived from porcine skin
■USP: Low endotoxin level (NMT 100 EU/g)
■Storage condition: Room temperature
■Expiration date: Two years from the date of manufacture
・Virus inactivated product
・High solubility in water
Product appearance
・Spongiform
・Size: approx.140x100 x10 mm
Applocation
・Scaffold
・Cell culture / transplantation
・Electrospinning etc.

Collagen FD

beMatrix product list

Product name Content
beMatrix Gelatin LS-H 10 g
beMatrix Gelatin LS-W 10 g
beMatrix Collagen AT 100 mL
beMatrix Collagen TE 100 mL
beMatrix Collagen FD 1 g
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For Research Use Only
The above products are sold for research purpose only. Regardless of purposes, such as production of drugs, product quality control, various diagnostic test, medical treatment, or other researches, do not use products for human body.