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Collagen for Cell Culture

Cellmatrix

Cellmatrix products have been used by a lot of researchers all over the world for over 30 years. Please choose optimal products according to your experiments.

Collagen for Cell Culture
How to choose Cellmatrix
Type I product

Collagen for
Three-dimensional
Culture/Collagen for Coating

★Collagen solution at pH 3 with a concentration of 3 mg/mL

■Storage condition: Cold storage (4 °C to 8 °C)

■Expiration date: One year from the date of manufacture

Type I-A

・Ideal for collagen gel culture.

・The gel has high transparency and ensures ease of
microscopic observation.

・Acid extraction of collagen derived from porcine tendons.

Type I-P

・Recommended for collagen gel culture and collagen-
coated culture.

・Forms a gel softer than Type I-A.

・Pepsin-solubilized collagen derived from porcine tendons.

Type I-C

・Recommended for collagen-coated culture.

・Pepsin-solubilized collagen derived from porcine skin.

Type III

・Recommended for collagen-coated culture.

・Not turning into a gel.

・Type III collagen derived from porcine skin.

Type IV

・Recommended for collagen-coated culture.

・Not turning into a gel.

・Type IV collagen derived from bovine lens capsules

・purified with pepsin treatment.

Growth morphology of mouse breast cancer cells cultured in an embedded collagen gel

Collagen Gel Culture Kit / Concentrated Culture Solution / Buffer Solution for Reconstitution

■Storage condition: Cold storage (4 °C to 8 °C)■Expiration date: In four months

Collagen Gel Culture Kit

Collagen Gel Culture Kit

■ Contents

Cellmatrix Type I-A

20 mLOne bottle

Concentrated culture solution (Ham’s F-12 and MEM)

5 mL eachOne bottle each

Buffer solution for reconstitution

4 mLOne bottle

Concentrated Culture Solution

Concentrated Culture Solution

Concentrated culture solution adjusted for collagen gel culture, Ham’s F-12 culture solution as a 10x concentrated product, MEM-Hanks’ culture solution, DF culture solution (DME:F-12=1:1), 199 culture solution, DME culture solution as 5x concentrated product, and RPMI-1640 culture solution are available.

Buffer Solution for Reconstitution

Buffer Solution for Reconstitution

Buffer solution for reconstitution adjusted for collagen gel culture

■Composition

Sodium hydroxide 50 mM
Sodium hydrogen carbonate 260 mM
HEPES 200 mM

Cellmatrix product list

Product name Content
Cellmatrix Type I-A 20 mL
100 mL
Cellmatrix Type I-P 20 mL
100 mL
Cellmatrix Type I-C 20 mL
100 mL
Cellmatrix Type III 5 mL
20 mL
100 mL
Cellmatrix Type IV 5 mL
20 mL
100 mL
Collagen gel culture kit 1 kit
Ham’s F-12 concentrated culture solution 100 mL
MEM-Hank’s concentrated culture solution 100 mL
DF concentrated culture solution 100 mL
199 concentrated culture solution 100 mL
DME concentrated culture solution 100 mL
RPMl-1640 concentrated culture solution 100 mL
Buffer solution for reconstitution 4 mL 15bottles

Method of Collagen Gel Culture

Prepare the following three types of solutions, i.e, A, B, and C.

A: Cellmatrix Type I-A or I-P
B: 10x concentrated culture solution1)
C: Buffer Solution for Reconstitution

Mix A and B well at a ratio of 8 to 1 while cooling A and B so that the mixture will not produce foam. Then add C at a ratio of 1 and mix them together.1)

1) In the case of using culture solution at a concentration of approximately 5x,
the following mixture ratio will apply: A:B:C=7:2:1

Culture on collagen gel

Culture on collagen gel

The above collagen mixture solution will turn into a gel
when the solution is dispensed to a culture dish and
heated at 37°C for 30 minutes after cooling the mixture.

Spray the cell dispersion onto the gel.
After the cells adhere to the gel, it will be possible to
culture the cells like normal monolayer culture.

Collagen gel-embedded culture

Collagen gel-embedded culture

Add cell pellets collected by centrifugation to the
above collagen mixture solution, and mix them with the
solution.

Dispensed this collagen mixture solution to a culture
dish and leave the solution at 37°C for 30 minutes so
that the solution will turn into a gel.

After the gel is formed, overlay with culture solution
and perform culture in an ordinary manner.

Method of Collagen Coating

Method of Collagen Coating

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For Research Use Only
The above products are sold for research purpose only. Regardless of purposes, such as production of drugs, product quality control, various diagnostic test, medical treatment, or other researches, do not use products for human body.