How to choose Cellmatrix Type I products
We provide multiple types of collagen products. Please select the optimum product for your experimental use (see Figure 1). Typical culture methods using collagen are as follows.
Collagen gel embedded culture (3D culture)
This is a method for three-dimensional cell culture in a collagen gel. In 3D culture, cells can be cultured in an environment extremely similar to the natural in vivo conditions.
Cellmatrix Type Ⅰ-A
Cellmatrix Type I-A is a collagen product with an optimized gelation rate and transparency for 3-dimensional culture. It can quickly form a gel before the cells sink to the bottom of the culture dish. In addition, the gel is highly transparent, allowing easy observation of the cells embedded in the collagen gel (Fig. 2).
Comparison of transparency
Collagen on-gel culture
This method involves cell culture on a collagen gel. Culturing cells on a collagen gel mimics an environment similar to the living body.
Recommended product: Cellmatrix Type I-A and Type I-P
Collagen coating culture
In this method, cells are cultured on substrates coated with collagen molecules to improve cell adhesion and activation of intracellular signals by contact with collagen molecules.
Recommended product: Cellmatrix Type I-C and Type I-P
How to choose according to your application
Features of Cellmatrix products
Each Cellmatrix product has a different gelling ability and viscosity. For culturing on a collagen gel, please use products with high gelation ability. Products with lower viscosity are suitable for coating culture because they are easier to handle. Please refer to the summarized table below.
Cellmatrix Type III and Type IV do not form a gel by themselves. However, they can be used to form a gel and carry out collagen gel culture by mixing them with Cellmatrix Type I-A. Stable gels can be formed if the ratio of Cellmatrix Type I-A is 40% or more.
|Type Ⅰ-A||Very good||Good|
|Type Ⅰ-C||Not good||Very good|
|Type Ⅲ||Not good||Very good|
|Type Ⅳ||Very good|
The above products are sold for research purpose only. Regardless of purposes, such as production of drugs, product quality control, various diagnostic test, medical treatment, or other researches, do not use products for human body.